Water Soluble Peroxyoxamide Reagent: Evaluation for Fluorescent Silica Nano- Particles Chemiluminescence
نویسندگان
چکیده
Enzymatic and nonenzymatic chemiluminescence immunoassay methods are widely used as an analytical tool in various bio-molecular detection applications. Here, we demonstrate the synthesis and 1 H NMR analysis of peroxyoxamide reagents and its application for characterization of covalently linked texas red dye with goat anti-mouse IgG antibody and generic goat IgG antibody on mesoporous silica-particles through luminometer and CCD imaging in (90%) aqueous solution. Further, fluorescein dye doped silica-nanoparticles (FSiNPs) were synthesized and characterized through SEM and TEM images. The function of F-SiNPs was analyzed in nanomolar range through chemilumineacense reaction of peroxyoxamide reagent with hydrogen peroxide in aqueous medium.
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